|
KMID : 1004820090100030125
|
|
Journal of Biomedical Research
2009 Volume.10 No. 3 p.125 ~ p.131
|
|
|
The Improved Effects for Ischemia of The Germinated Rough Rice (Oryza sativa) Extracts on Mouse Hindlimb Ischemia Model
|
|
Shim Tae-Jin
Kim Tae-Myoung
Na Ba-Ul
Bae Min-Gu
Kim Hyun-Young
Jeong Heon-Sang
Kim Dae-Joong
|
|
|
Abstract
|
|
|
|
Germinated rough rice (GRR) has many healthy effects such as antioxidant activity, inhibitionof chronic inflammatory disease and prevention of vascular disease. In this study, germinated Korean ricesample which was planted in 2008 from Korean Rural Development Administration was used. The purposeof this study was to recognize the effect of the ethanol extract of GRR on the ischemic damage of skeletalmuscle. Right femoral artery ligation was performed to induce ischemic condition in mice and the mixedbasal diet with the ethanol extract of GRR at various levels (100, 200, and 400 mg/kg diet) was given for2 weeks. After the mice were sacrificed, the hind limb muscle samples of the mice were fixed, sectioned,stained with Haematoxylin-Eosin (H&E), Masson’s trichrome, and performed for Immunohistochemistry(IHC) for caspase-3. In histopathological findings of H&E and Masson’s trichrome stain, the treatmentof GRR at the level of 400 mg/kg diminished fibrosis and necrosis in the muscle compared to the control. The expression of caspase-3 protein was also decreased by the treatment of GRR at the level of 400 mg/kg compared to the control. These results suggest that the ethanol extract of GRR may have a protective effect against muscle damage during the ischemia in skeletal muscle of hindlimb.
|
|
|
KEYWORD
|
|
|
Germinated rough rice (GRR), Hindlimb muscle, Ischemia, Caspase-3
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|